Sintesis selulosa bakteri dari jerami kulit nangka dengan penambahan beberapa konsentrasi sukrosa

Synthesis of bacterial cellulose from jackfruit skin straw with the addition of several concentrations of sucrose

  • Budi Santosa Program Studi Teknologi Industri Pertanian, Fakultas Pertanian, Universitas Tribhuwana Tunggadewi, Jawa Timur
  • Lorine Tantalu Program Studi Teknologi Industri Pertanian, Fakultas Pertanian, Universitas Tribhuwana Tunggadewi, Jawa Timur
  • Natanael Woleka Sairo Mahasiswa Program Studi Teknologi Industri Pertanian, Fakultas Pertanian, Universitas Tribhuwana Tunggadewi, Jawa Timur
Keywords: bacterial cellulose, jackfruit skin straw, sucrose

Abstract

Introduction: Bacterial cellulose or also called nata is a drink shaped like a gel, white, consisting of cellulose (dietary fiber) made from a liquid medium containing carbohydrates by fermenting using the help of microbes with the name Acetobacter xylinum. The purpose of this study was to obtain the best concentration of sucrose in bacterial cellulose synthesis from jackfruit skin straw. Methods: The experimental design used in this study is a complete randomized design of a single factor, as a factor in the concentration of sucrose consisting of 5 levels namely K1 = 1%, K2 = 1.5%, K3 = 2%, K4 = 2.5% and K5 = 3%. Each treatment is repeated 5 times so that the total sample observed as many as 25 pieces. The observed parameters are the thickness of the nata, the weight of the nata, the total fiber content of the nata and the beginning of the formation of nata. Parameters of nata thickness, weight of nata and total fiber content of nata were observed on the 14th day of fermentation after harvesting nata. The initial parameters of the formation of nata are observed at the beginning of fermentation. Results: The results showed that nata thickness increased at 1%, 1.5% and 2% sucrose concentrations. The highest weight of jackfruit skin straw nata was found in the treatment with 2% sucrose concentration, which was 215.8 grams. The sucrose concentration of 2% resulted in the highest fiber content when compared to other treatments. The initial formation of jackfruit skin straw nata was the fastest at the 2% sucrose concentration treatment, which was 2.2 days. Conclusion: The best bacterial cellulose (nata) of jackfruit skin straw was obtained from the treatment with 2% sucrose concentration.

References

Barry, V., & Mc Cleary. (2014). Modification to AOAC official mehods 2009.01 and 2011.25 to allow for minor overestimation of low moleculer weight soluble dietary fiber in samples containing starch. Journal of AOAC International, 97(3), 896-901.

Direktorat Jenderal Hortikultura Kementerian Pertanian Republik Indonesia. (2019). Produktivitas nasional buah lima tahun terakhir. Kementerian Pertanian - Data Lima Tahun Terakhir. Diakses 15 Januari 2021.

Fivien, W., Kumalaningsih, S., & Effendi, E.. (2012). Pengaruh penambahan sukrosa dan asam asetat glacial terhadap kualitas nata dari whey tahu dan substrat air kelapa. Industria: Jurnal Teknologi dan Manajemen Agroindustri, 1(2), 86-93.

Gayathry, G. (2015). Production of nata de coco a natural dietary fibre product from mature coconut water using GluconAcetobacter xylinum. International Journal Food Fermentation Technology, 5(2), 231-235.

Hamad, A. & Kristiono. (2013). Pengaruh penambahan sumber nitrogen terhadap hasil fermentasi nata de coco. Majalah Ilmiah Momentum, 9(1), 62-65.

Hamad, A., Andriyani, N. A., Wibisono, H., & Sutopo, H. (2011). Pengaruh penambahan sumber karbon terhadap kondisi fisik nata de coco. Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto), 12(2), 74-77.

Hanafiah, K.A. (2012). Rancangan percobaan (teori dan aplikasi). Jakarta: Rajawali Press.

Herawaty, N., & Moulina, M. A. (2015). Kajian variasi konsentrasi sukrosa terhadap karakteristik nata timun suri (Cucumis sativus L.). AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian, 2(2), 89-104.

Hidayat, N., Masdiana, C., Padaga & Suhartini, S. (2006). Mikrobiologi industri. Yogyakarta: Penerbit Andi.

Kumalaningsih, S. (2012). Metodologi penelitian (kupas tuntas cara mencapai tujuan). Malang: UB Press.

Majesty, J., Argo, B. D., & Nugroho, W. A. (2014). Pengaruh penambahan sukrosa dan lama fermentasi terhadap kadar serat nata dari sari nanas (nata de pina). Jurnal Keteknikan Pertanian Tropis dan Biosistem, 3(1), 80-85.

Naufalin, R., & Wibowo, C. (2004). Pemanfaatan hasil samping pengolahan tepung tapioka untuk pembuatan nata de cassava: kajian penambahan sukrosa dan ekstrak kecambah. Jurnal Teknologi Industri Pangan, 15(2), 153-158.

Nur'aini, H., & Sari, E. R. (2016). Identifikasi mutu nata kulit buah naga (Hylocereus undatus) dengan variasi konsentrasi sukrosa. AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian, 3(1), 165-174.

Nurhayati, S. (2006). Kajian pengaruh kadar gula dan lama fermentasi terhadap kualitas nata de soya. Jurnal Matematika Sains dan Teknologi, 7(1), 40-47.

Oliveira, P. M., Brosnan, B., Furey, A., Coffey, A., Zannini, E., & Arendt, E. K. (2015). Lactic acid bacteria bioprotection applied to the malting process. Part I: Strain characterization and identification of antifungal compounds. Food Control, 51, 433-443.

Pambayun, R.(2002). Teknologi pengolahan nata de coco. Yogyakarta: Penerbit Kanisius.

Pratiwi,E. (2002). Karakteristik nata dari pulp kakao mulia (Theobroma cacao L.) dengan penambahan berbagai konsentrasi sukrosa. Jurnal Tekologi Pangan dan Hasil Pertanian, 5(2), 81-85.

Ratnawati, D. (2007). Kajian variasi kadar glukosa dan derajat keasaman (pH) pada pembuatan nata de citrus dari jeruk asam (Citrus limon. L). GRADIEN, 3(2), 257-261.

Rizal, H. M., Pandiangan, D. M., & Saleh, A. (2013). Pengaruh penambahan gula, asam asetat dan waktu fermentasi terhadap kualitas nata de corn. Jurnal Teknik Kimia, 19(1), 34-39.

Rose, D., Puji Ardiningsih & Nora Idiawati. (2018). Karakteristik nata de jackfruit (Artocarpus heterophyllus) dengan variasi konsentrasi starter Acetobacter xylinum. Jurnal Kimia Khatulistiwa, 7(4), 1-7.

Santosa, B. (2020c). Proses pembuatan bubuk probiotik lactobacillus plantarum menggunakan filler bacterial cellulose [Disertasi]. Universitas Brawijaya Malang.

Santosa, B., & Wirawan, W. (2021b). Evaluasi sifat fisika, kimia dan kandungan logam berat di dalam nata de coco yang dibuat menggunakan sumber nitrogen dari NPK. Teknologi Pangan: Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian, 12(2), 250-256.

Santosa, B., Ahmadi, K., & Taeque, D. (2012). Dextrin concentration and carboxy methyl cellulosa (cmc) in making of fiber-rich instant baverage from nata de coco. IEESE International Journal of Science and Technology, 1(1), 6.

Santosa, B., Rozana, R., & Astutik, A. (2021a). Pemanfaatan sumber nitrogen organik dalam pembuatan nata de coco. Teknologi Pangan: Media Informasi Dan Komunikasi Ilmiah Teknologi Pertanian, 12(1), 52-60.

Santosa, B., Tantalu, L., & Sugiarti, U. (2019a). Penambahan ekstrak kulit buah naga pada pengembangan produk nata de coco berantioksidan. Teknologi Pangan: Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian, 10(1), 1-8.

Santosa, B., Wignyanto, W., Hidayat, N. & Sucipto,S. (2020a). Optimization of NaOH concentration and trichloroacetic acid in bacterial carboxymethylation cellulose. Food Research Journal, 4(3), 594-601

Santosa, B., Wignyanto, W., Hidayat, N., & Sucipto, S. (2020b). The quality of nata de coco from sawarna and mapanget coconut varieties to the time of storing coconut water. Food Research Journal, 4(4), 957-963.

Santosa, B., Wirawan, W., & Muljawan, R. E. (2019). Pemanfaatan molase sebagai sumber karbon alternatif dalam pembuatan nata de coco. Teknologi Pangan: Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian, 10(2), 61-69.

Sopandi, T., & Wardah. (2014). Mikrobiologi pangan. Yogyakarta: Penerbit Andi.

Tamimi, A., Sumardi HS., & Hendrawan, Y. (2015). Pengaruh penambahan sukrosa dan urea terhadap karakteristik nata de soya asam jeruk nipis – in press. Jurnal Bioproses Komoditas Tropis, 3(1), 1-10.

Published
2022-03-31
How to Cite
Santosa, B., Tantalu, L., & Sairo, N. W. (2022). Sintesis selulosa bakteri dari jerami kulit nangka dengan penambahan beberapa konsentrasi sukrosa. AGROMIX, 13(1), 67-73. https://doi.org/10.35891/agx.v13i1.2881
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Articles