Karakterisasi kandungan vitamin B12, folat dan isoflavon tempe kacang kedelai sebagai bahan pangan fungsional
Characterization of Vitamin B12, Folate and Isoflavones of Soybean Tempeh as Functional Food
Abstract
Tempeh is a fermented food ingredient from soybeans that uses various Rhizopus molds, such as Rhizopus oligosporus, Rhizopus oryzae, Rhizophus stolonifer. In this study, the preparation of tempe using mixed culture was carried out in the ratio of Rhizopus oryzae, Rhizopus oligosporus, Rhizopus stolonifer inoculums, namely 1: 1: 1; 1: 2: 1; 2: 1: 2; 1: 1: 2; 2: 1: 1; market yeast "Raprima", produces the characteristics of tempe according to SNI 01-3144-2009, formation of Vitamin B12, Folate and Isoflavones as well as pH, and Total Bacteria. The content of vitamin B12 was obtained in yeast "raprima" of 0.013 g / 50g of tempeh while tempeh made with pure inoculums of Rhizopus sp did not contain vitamin B12 or folic acid, pH value of 4.2 and total bacteria 14.3x108 cfu / ml and organoleptic reception. Tempe 1: 2: 1 has the characteristics of color, aroma, taste, texture and viscosity acceptable to the panelists.
References
AOAC International. (2006). Official methods of analysis of aoac international. Aoac, February, 3172. https://www.techstreet.com/standards/official-methods-of-analysis-of-aoac-international-20th-edition-2016?product_id=1937367
Asmoro, N. W. (2016). Asam folat pada fermentasi tempe kedelai hitam. Jurnal Ilmiah Teknosains, 2(1), 66–72.
Astawan, M., Mardhiyyah, Y. S., & Wijaya, C. H. (2018). Potential of bioactive components in tempe for the treatment of obesity. Jurnal Gizi dan Pangan, 13(2), 79–86. https://doi.org/10.25182/jgp.2018.13.2.79-86
Balarabe, M.M, Mohammed, S.S.D., & Orukotan, A. . (2017). Physico-chemical analysis and sensory evaluation of bread produced using different indigenous yeast isolates. Science World Journal, 12(1597–6343), 33–37.
Barus, T., Suwanto, A., & Agustina, W. (2010). Analisis Matagenom Komunitas Bakteri Tempe dengan Teknik Terminal Restriction Fragment Length Polymorphism ( T-RFLP ) Metagenomic Analysis of Bacterial Diversity in Tempe using Terminal Restriction Fragment Length Polymorphism ( T-RFLP ) Technique. 15(April), 2.
Barus, T., Suwanto, A., Tri wahyudi, A., & Wijaya, H. (2008). Role of bacteria in tempe bitter taste formation: microbiological and molecular biological analysis based on 16s rrna gene. Microbiology Indonesia, 2(1), 17–21. https://doi.org/10.5454/mi.2.1.4
Cahyadi, W. (2006). Kedelai Khasiat dan teknologi. Bumi Aksara.
Cao, Z. H., Green-Johnson, J. M., Buckley, N. D., & Lin, Q. Y. (2019). Bioactivity of soy-based fermented foods: A review. Biotechnology Advances, 37(1), 223–238. https://doi.org/10.1016/j.biotechadv.2018.12.001
FAO/WHO Expert Consultation. (2005). Human Vitamin and Mineral Requirements. Geneva : World Health Organization, 2nd ed, 341 p. http://apps.who.int/iris/handle/10665/42716
Feng, X.M., T. O. L., & Schnurer, J. (2006). Production of volatile compounds by rhizopus oligosporus during soybean and barley tempeh fermentation. Journal of Food Microbiology, 113, 133–141.
Handoyo, T., & Morita, N. (2006). Structural and functional properties of fermented soybean (Tempeh) by using rhizopus oligosporus. International Journal of Food Properties, 9(2), 347–355. https://doi.org/10.1080/10942910500224746
Jeleń, H., Majcher, M., Ginja, A., & Kuligowski, M. (2013). Determination of compounds responsible for tempeh aroma. Food Chemistry, 141(1), 459–465. https://doi.org/10.1016/j.foodchem.2013.03.047
Jennessen, J., Schnürer, J., Olsson, J., Samson, R. A., & Dijksterhuis, J. (2008). Morphological characteristics of sporangiospores of the tempe fungus Rhizopus oligosporus differentiate it from other taxa of the R. microsporus group. Mycological Research, 112(5), 547–563. https://doi.org/10.1016/j.mycres.2007.11.006
Kanti, A.-. (2017). Potensi dari kapang aspergilus niger, rhizophus oryzae dan neurospora sitophila sebagai penghasil ezim fitase dan amilase pada substrate ampas tahu. Buletin Peternakan, 41(1), 26. https://doi.org/10.21059/buletinpeternak.v41i1.13337
Kustyawati, M. E., Subeki, Murhadi, Rizal, S., & Astuti, P. (2020). Vitamin B12 production in soybean fermentation for tempeh. AIMS Agriculture and Food, 5(2), 262–271. https://doi.org/10.3934/AGRFOOD.2020.2.262
Maryati, Y., Susilowati, A., Melanie, H., & Lotulung, P. D. (2019). Fermentation of soybean (Glycine max (l.) merr) using mix inocula of Rhizopus sp. and Sacharomycescereviceae for alternative source of folic acid. IOP Conference Series: Materials Science and Engineering, 536(1). https://doi.org/10.1088/1757-899X/536/1/012124
Moa, H., Kariluoto, S., Piironen, V., Zhu, Y., Sanders, M. G., Vincken, J. P., Wolkers-Rooijackers, J., & Nout, M. J. R. (2013). Effect of soybean processing on content and bioaccessibility of folate, vitamin B12 and isoflavones in tofu and tempe. In Food Chemistry (Vol. 141, Issue 3, pp. 2418–2425). https://doi.org/10.1016/j.foodchem.2013.05.017
Morsy O.M., Sharoba, A. M., Bahlol, H., & Abdel-mawla, E. (2014). Production and evaluation of extruded food products by using spirulina algae. Annals of Agric. Sci., Moshtohor, 52(4), 329–342.
Negara, J. K., Sio, A. K., Rifkhan, R., Arifin, M., Oktaviana, A. Y., Wihansah, R. R. S., & Yusuf, M. (2016). Aspek mikrobiologis, serta Sensori (Rasa, Warna,Tekstur, Aroma) Pada Dua Bentuk Penyajian Keju yang Berbeda. Jurnal Ilmu Produksi Dan Teknologi Hasil Peternakan, 4(2), 286–290. https://doi.org/10.29244/jipthp.4.2.286-290
Priyanka, P., & Jaya Raju, K. (2013). Neutral protease production by Rhizopus oligosporus NCIM 1215 under solid state fermentation using mixed substrates of agro industrial residues. International Journal of Chemical Sciences, 11(1), 291–305.
Shao-Quan, L; Marlene, T. (2009). Enchancement of survival of probiotic and non-probiotic lactic acid bacteria by yeast in fermented milk under non-refrigerated condition. International Journal of Food Microbiology, 135, 34–38.
Sine, Y., & Soetarto, E. S. (2018). Perubahan Kadar Vitamin Dan Mineral Pada Fermentasi Tempe Gude (Cajanus cajan L.). Jurnal Saintek Lahan Kering, 1(1), 1–3. https://doi.org/10.32938/slk.v1i1.414
Sulistyarsi, A., Pujiati, & Erviyanti, D. (2018). Uji Kandungan Protein Dan Uji Organoleptik Terhadap Kualitas Tempe berbahan dasar kacang-kacangan. Prosiding Seminar Nasional SIMBIOSIS I, June, 161–165.
Vital, R. J., Bassinello, P. Z., Cruz, Q. A., Carvalho, R. N., De Paiva, J. C. M., & Colombo, A. O. (2018). Production, quality, and acceptance of tempeh and white bean tempeh burgers. Foods, 7(9). https://doi.org/10.3390/foods7090136
Winanti, R. (2014). Higienitas Produk Tempe Berdasarkan Perbedaan Metode Inokulasi. Unnes Journal of Life Science, 3(1), 39–46.
Wolkers – Rooijackers, J. C. M., Endika, M. F., & Smid, E. J. (2018). Enhancing vitamin B12 in lupin tempeh by in situ fortification. Lwt, 96(September 2017), 513–518. https://doi.org/10.1016/j.lwt.2018.05.062
Zhang, S. J., De Bruyn, F., Pothakos, V., Contreras, G. F., Cai, Z., Moccand, C., Weckx, S., & De Vuyst, L. (2019). Influence of Various Processing Parameters on the Microbial Community Dynamics, Metabolomic Profiles, and Cup Quality During Wet Coffee Processing. Frontiers in Microbiology, 10(November), 1–24. https://doi.org/10.3389/fmicb.2019.02621
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