Virulensi beberapa isolat Pantoea ananatis penyebab penyakit hawar daun bakteri (bacterial leaf blight) pada varietas bawang merah
The virulence of several isolates of Pantoea ananatis causes bacterial leaf blight in shallot varieties
DOI:
https://doi.org/10.35891/agx.v11i2.1946Keywords:
leaf blight bacterial, isolate, Pantoea ananatis, virulentAbstract
Bacterial leaf blight caused by Pantoea ananatis in onions is a new disease in Indonesia. Information regarding the level of virulence of these pathogens in onion plants is unknown. This study aims to determine the level of virulence of P. ananatis isolates in onion plants originating from Cirebon, Tegal, Nganjuk, Bantul, and Sigi districts. The study was conducted by an experimental method using 150 pathogen isolates and 4 onion cultivars in a greenhouse from 5 regions. Experimental data were analyzed descriptively with the help of image and table views. The results showed that all pathogenic isolates had the same level of virulence (uniform) and were very virulent against 4 cultivars of obese bima, blue rice fields, bauji, and palasa. All of these isolates gave the same response to the fast incubation period (2.35 - 2.90 days), the percentage of leaves that were heavily attacked (67.44 - 81.76%), and a high infection rate (> 0.5 units per day).
References
Agrios, N. G. (2005). Plant Pathology (5 ed.). University of Florida.
Alippi, A. M., & López, A. C. (2010). First report of leaf spot disease of maize caused by Pantoea ananatis in Argentina. Plant Disease, 94(4), 487. https://doi.org/10.1094/PDIS-94-4-0487A
Asrul, B. Hadisutrisno, T. Arwiyanto, & J. Widada (2014). Peranan faktor lingkungan terhadap penyakit hawar daun bakteri (Pantoea ananatis) pada tanaman bawang merah. hlm 1–12. Seminar Nasional Perhimpunan Fitopatologi Indonesia (PFI) Komda Yogyakarta, Solo dan Semarang, 20 September. Yogyakarta.
Asrul, B. Hadisutrisno, T. Arwiyanto, & J. Widada (2013). The spread of bacterial leaf blight disease at production centers of shallot in indonesia. Biota, 18(1), 27−36
Asrul & Umrah (2019). Host range Pantoea ananatis the causal agent of bacterial leaf blight on Allium spp. Agroland: The Agricultural Sciences Journal, 6(1), 27–33.
Billing, E. (1982). Entry and establishment of pathogenic bacteria in plant tissues. Symposium Series - Society for Applied Bacteriology. http://agris.fao.org/agris-search/search.do? recordID=US201301987106
Boch, J., & Bonas, U. (2001). Gram-Negative Plant Pathogenic Bacteria. Milhldorfer I, Schafer KP (eds): Emerging bacterial pathogens. Contrib Microbiol. Basel, Karger, 8, 186–196.
Brugman, E., Purajanti, E. D., & Fuskhah, E. (2017). Pengendalian penyakit hawar (late blight) pada kentang (Solanum tuberosum l.) melalui penerapan solarisasi tanah dan aplikasi agen hayati Trichoderma harzianum [Disertasi]. Fakultas Peternakan dan Pertanian Undip.
Buonaurio, R. (2008). Infection and plant defense responses during plant-bacterial interaction. Plant-microbe interactions, 169–197.
Carr, Eric A., Zaid, A. M., Bonasera, J. M., Lorbeer, J. W., & Beer, S. V. (2013). Infection of onion leaves by Pantoea ananatis leads to bulb infection. Plant Disease, 97(12), 1524–1528. https://doi.org/10.1094/PDIS-06-12-0597-RE
Diab, S., Bashan, Y., Okon, Y., & Henis, Y. (1982). Effects of relative humidity on bacterial scab caused by Xanthomonas campestris pv. vesicatoria on pepper. Phytopathology, 72(9), 1257–1260.
Dutta, B., Anderson, F., Smith, S., & Gitaitis, R. D. (2017). Epiphytic Survival of Pantoea ananatis on Richardia scabra L. in Georgia. Plant Disease, 101(4), 613–618. https://doi.org/10.1094/PDIS-10-16-1411-RE
Ezepchuk, Y. V. (2013). Biology of pathogenicity (theoretical review). Annual Research & Review in Biology, 3(4), 805–813.
Gabriel, D. W. (1999). Why do pathogens carry avirulence genes? Physiological and Molecular Plant Pathology, 55, 205–214.
Goszczynska, T., Botha, W. J., Venter, S. N., & Coutinho, T. A. (2007). Isolation and identification of the causal agent of brown stalk rot, a new disease of maize in South Africa. Plant Disease, 91(6), 711–718. https://doi.org/10.1094/PDIS-91-6-0711
Goszczynska, T., Moloto, V. M., Venter, S. N., & Coutinho, T. A. (2006). Isolation and identification of Pantoea ananatis from onion seed in South Africa. Seed Science and Technology, 34(3), 655–668. https://doi.org/10.15258/sst.2006.34.3.12
Guest, D., & Brown, J. (1997). Plant defences against pathogens. Dalam Plant pathogens and plant diseases. Rockvale Publications for the Division of Botany, Rockvale Publications for the Division of Botany, University of New England
Habazar, T., & Rivai, F. (2004). Bakteri patogenik tumbuhan. Andalas University Press.
Janse, J. D. (2005). Phytobacteriology: Principles and practice. CABI.
Kim, J., Choi, O., & Kim, T.-S. (2012). An outbreak of onion center rot caused by Pantoea ananatis in Korea. Plant Disease, 96(10), 1576. https://doi.org/10.1094/PDIS-03-12-0251-PDN
Kim, M. K., Ryu, J. S., Lee, Y. H., & Yun, H. D. (2007). First report of Pantoea spp. Induced soft rot disease of Pleurotus eryngii in Korea. Plant Disease, 91(1), 109–113. https://doi.org/10.1094/PD-91-0109A
McDonald, M. R., de los Angeles Jaime, M., & Hovius, M. H. Y. (2004). Management of diseases of onions and garlic. Dalam S. A. M. H. Naqvi (Ed.), Diseases of Fruits and Vegetables: Volume II: Diagnosis and Management (hlm. 149–200). Springer Netherlands. https://doi.org/10.1007/1-4020-2607-2_6
Morohoshi, T., Nakamura, Y., Yamazaki, G., Ishida, A., Kato, N., & Ikeda, T. (2007). The plant pathogen Pantoea ananatis produces Nacylhomoserine lactone and causes center rot disease of onion by quorum sensing. Journal of Bacteriology, 189(22), 8333–8338. https://doi.org/10.1128/JB.01054-07
Nunez, J. J., Gilbertson, R. L., Meng, X., & Davis, R. M. (2002). First report of Xanthomonas leaf blight of onion in California. Plant Disease, 86(3), 330–335. https://doi.org/10.1094/ PDIS.2002.86.3.330B
Oka, I. N. (1993). Pengantar epidemiologi penyakit tanaman. Gadjah Mada University Press.
Ormsby, M., & Pottinger, B. (2009). Import Risk Analysis: Onion (Allium cepa Liliaceae) Fresh Bulbs for Consumption from China (12 ed., Vol. 12). Ministry of Agriculture and Forestry Te Manatu Ahuwhenua, Ngaherehere.
Raju, J., Benagi, V. I., Naragund, V. B. & Ashtaputre, A. 2011. Survey for the incidence and severity of bacterial blight in pomegranate caused by Xanthomonas axonopodis pv. punicae Karnataka. Journal of Agricultural Science, 24(4), 570 – 572.
Resti, Z., Habazar, T., Putra, D. P., & Nasrum. (2016). Aktivitas enzim peroksidase bawang merah yang diintroduksi dengan bakteri endofit dan tahan terhadap penyakit hawar daun bakteri (Xanthomonas axonopodis pv. allii). Jurnal Hama dan Penyakit Tumbuhan Tropika, 16(2), 131–137. https://doi.org/10.23960/j.hptt.216131-137
Schroeder, B. K., Schwartz, H. F., Schwartz, H. F., & du Toit, L. J. (2011). Bacterial Diseases. Onion ipmPIPE Diagnostic Pocket Series. http://mtvernon.wsu.edu/path_team/Onion-Card-Bacterial-Diseases.pdf
Schumann, G., & D’Arcy, C. (2010). Essential plant pathology. 2 a Edición (2 ed.). ST. Paul, Minnesota (USA) American Phytopathological Society Press.
Schwartz, H. F., Otto, K. L., & Gent, D. H. (2003). Relation of temperature and rainfall to development of Xanthomonas and Pantoea leaf blights of onion in Colorado. Plant Disease, 87(1), 11–14. https://doi.org/10.1094/PDIS.2003.87.1.11
Shin, G. Y., Schachterle, J. K., Shyntum, D. Y., Moleleki, L. N., Coutinho, T. A., & Sundin, G. W. (2019). Functional Characterization of a Global Virulence Regulator Hfq and Identification of Hfq-Dependent sRNAs in the Plant Pathogen Pantoea ananatis. Frontiers in Microbiology, 10(2075), 1-19. https://doi.org/10.3389/fmicb.2019.02075
Soesanto, L., & Rahayunia, R. F. (2011). Pengimbasan ketahanan bibit pisang ambon kuning terhadap penyakit layu fusarium dengan beberapa jamur antagonis. Jurnal Hama dan Penyakit Tumbuhan Tropika, 9(2), 130–140. https://doi.org/Doi: http://dx.doi.org/10.23960/j.hptt.29130-140
Staskawicz, B. J., Ausubel, F. M., Baker, B. J., Ellis, J. G., & Jones, J. D. (1995). Molecular genetics of plant disease resistance. Science, 268(5211), 661-667.
Stice, S., Dutta, B., & Kvitko, B. H. (2019). A plasmid gene cluster makes major contributions to Pantoea ananatis virulence on onion by conferring tolerance to a reactive sulfur phytoanticipin. Plant Health 2019. https://apsnet.confex.com/apsnet/2019/ meetingapp.cgi/Paper/14373
Stice, S. P., Stumpf, S. D., Gitaitis, R. D., Kvitko, B. H., & Dutta, B. (2018). Pantoea ananatis genetic diversity analysis reveals limited genomic diversity as well as accessory genes correlated with onion pathogenicity. Frontiers in Microbiology, 9(184), 1-18. https://doi.org/10.3389/fmicb.2018.00184
Surico, G. (2013). The concepts of plant pathogenicity, virulence/avirulence and effector proteins by a teacher of plant pathology. Phytopathologia Mediterranea, 52(3), 399–417.
Vahlingâ€Armstrong, C., Dung, J. K. S., Humann, J. L., & Schroeder, B. K. (2016). Effects of postharvest onion curing parameters on bulb rot caused by Pantoea agglomerans, Pantoea ananatis and Pantoea allii in storage. Plant Pathology, 65(4), 536–544. https://doi.org/10.1111/ppa.12438
Vander der Plank, J. E. (1963). Plant diseases: epidemic and control. Academic Press.
Wellerâ€Stuart, T., Toth, I., Maayer, P. D., & Coutinho, T. (2017). Swimming and twitching motility are essential for attachment and virulence of Pantoea ananatis in onion seedlings. Molecular Plant Pathology, 18(5), 734–745. https://doi.org/10.1111/mpp.12432
Zaid, A. M., Bonasera, J. M., & Beer, S. V. (2012). OEM—A new medium for rapid isolation of onion-pathogenic and onion-associated bacteria. Journal of Microbiological Methods, 91(3), 520–526. https://doi.org/10.1016/j.mimet.2012.09.031
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