Efek cahaya LED merah dan biru pada pertumbuhan, hasil dan kandungan klorofil tanaman pakcoy (Brassica chinensis L.) dalam Growbox

The Effects of red and blue LED light on growth, yield and chlorophyll content of pakchoy (Brassica chinensis L.) plants in growbox

Authors

  • Rosyida Laboratorium Fisiologi dan Pemuliaan Tanaman, Departemen Pertanian, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Kota Semarang
  • Karno Karno Laboratorium Fisiologi dan Pemuliaan Tanaman, Departemen Pertanian, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Kota Semarang
  • Fajrin Pramana Putra Laboratorium Fisiologi dan Pemuliaan Tanaman, Departemen Pertanian, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Kota Semarang
  • Julian Christopher Limantara Program Studi Agroekoteknologi, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Jalan Prof. Soedarto S.H Tembalang, Kota Semarang

DOI:

https://doi.org/10.35891/agx.v13i2.3028

Keywords:

LEDs, Light quality, Pakchoy, Chlorophyll

Abstract

Introduction: Light-emitting diodes (LEDs) are artificial light sources that have been widely used in indoor farming systems. The light quality of red and blue LEDs plays a role in plant growth and diverse physiological responses. The right ratio of red and blue LEDs is expected to increase the growth, yield and physiology of Pakchoy plants. Experiments on the effect of LED light quality on growth, yield and physiology of Pakchoy plants have been carried out at the Grow Box. Methods: The experiment was arranged in a Completely Randomized Design (CRD) consisting of 5 types of LED light quality, with the ratio of Red and Blue light (R:B), namely: (R10), (B10), (R5:B5), (R3:B7) , and (R7:B3). Each treatment was repeated 3 times, so there were 15 experimental units. Parameters observed included growth, yield and physiological components related to photosynthesis, namely: plant height (cm), plant length (cm), number of leaves (plant-1), leaf area (cm2), plant fresh weight (g plants -1), content of chlorophyll a, b and total (mg g-1), leaf carotenoids content (mg g-1), chlorophyll content of SPAD (Soil Plant Analysis Development), and DCGI (Dark Color Green Index). Results: The light quality of monochromatic blue LEDs (B10) and the combination of red and blue (R3:B7, R5:B5 and R7:B3) significantly improves vegetative growth, chlorophyll content, carotenoids and fresh weight than monochromatic red light (R10). Monochromatic red light R10 decreased growth, chlorophyll component and yield in Pakchoy plants. Conclusion: Red and blue LEDs with a ratio of R3:B7 showed the best results on all observation parameters.

References

Ahn, S. Y., Kim, S. A., & Yun, H. K. (2015). Inhibition of Botrytis cinerea and accumulation of stilbene compounds by light-emitting diodes of grapevine leaves and differential expression of defense-related genes. European journal of plant pathology, 143(4), 753-765.

Chang, S., Chunxia, L., Xuyang, Y., Song, C., Xuelei, J., Xiaoying, L., ... & Rongzhan, G. (2016). Morphological, photosynthetic, and physiological responses of rapeseed leaf to different combinations of red and blue lights at the rosette stage. Frontiers in Plant Science, 7, 1144. https://doi.org/10.3389/fpls.2016.01144.

Chen, X. L., Yang, Q. C., Song, W. P., Wang, L. C., Guo, W. Z., & Xue, X. Z. (2017). Growth and nutritional properties of lettuce affected by different alternating intervals of red and blue LED irradiation. Scientia Horticulturae, 223, 44-52.

Hogewoning, S. W., Trouwborst, G., Maljaars, H., Poorter, H., van Ieperen, W., & Harbinson, J. (2010). Blue light dose–responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. Journal of experimental botany, 61(11), 3107-3117. https://doi.org/10.1093/jxb/erq132

Jing, X., Wang, H., Gong, B., Liu, S., Wei, M., Ai, X., ... & Shi, Q. (2018). Secondary and sucrose metabolism regulated by different light quality combinations involved in melon tolerance to powdery mildew. Plant Physiology and Biochemistry, 124, 77-87. https://doi.org/10.1016/j.plaphy.2017.12.039

Kami, C., Lorrain, S., Hornitschek, P., & Fankhauser, C. (2010). Light-regulated plant growth and development. Current topics in developmental biology, 91, 29-66.

Kasim, M. U., & Kasim, R. (2012). Color changes of fresh-cut Swiss chard leaves stored at different light intensity. American Journal of Food Technology, 7(1), 13-21. https://doi.org/10.3923/ajft.2012.13.21

Ling, Q., Huang, W., & Jarvis, P. (2011). Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Photosynthesis research, 107(2), 209-214. https://doi.org/10.1007/s11120-010-9606-0

Liu, X., Guo, S., Chang, T., Xu, Z., & Takafumi, T. (2012). Regulation of the growth and photosynthesis of cherry tomato seedlings by different light irradiations of light emitting diodes (LED). African Journal of Biotechnology, 11(22), 6169-6177. https://doi.org/10.5897/AJB11.1191

Mehdi, S., Sasan, A., Mostafa, A., Mahboobeh, Z. M., Aida, S., Dimitrios, F., Tao, L. & Ernst, W. (2021). Monochromatic red light during plant growth decreases the size and improves the functionality of stomata in chrysanthemums. Functional Plant Biology, 48(5), 515-528. https://doi.org/10.1071/FP20280

Miao, Y., Chen, Q., Qu, M., Gao, L., & Hou, L. (2019). Blue light alleviates red light syndrome by regulating chloroplast ultrastructure, photosynthetic traits and nutrient accumulation in cucumber plants. Scientia Horticulturae, 257, 108680.

Paradiso, R., Meinen, E., Snel, J. F., De Visser, P., Van Ieperen, W., Hogewoning, S. W., & Marcelis, L. F. (2011). Spectral dependence of photosynthesis and light absorptance in single leaves and canopy in rose. Scientia Horticulturae, 127(4), 548-554.

Petrovska, R., Brang, P., Gessler, A., Bugmann, H., & Hobi, M. L. (2021). Grow slowly, persist, dominate—Explaining beech dominance in a primeval forest. Ecology and Evolution, 11(15), 10077-10089.

Rehman, M., Ullah, S., Bao, Y., Wang, B., Peng, D., & Liu, L. (2017). Light-emitting diodes: whether an efficient source of light for indoor plants?. Environmental Science and Pollution Research, 24(32), 24743-24752.

Savvides, A., Fanourakis, D., & van Ieperen, W. (2012). Coordination of hydraulic and stomatal conductances across light qualities in cucumber leaves. Journal of Experimental Botany, 63(3), 1135-1143. https://doi.org/10.1093/jxb/err348

Singh, D., Basu, C., Meinhardt-Wollweber, M., & Roth, B. (2015). LEDs for energy efficient greenhouse lighting. Renewable and Sustainable Energy Reviews, 49, 139-147. https://doi.org/10.1016/j.rser.2015.04.117.

Stahl, W., & Sies, H. (2003). Antioxidant activity of carotenoids. Molecular aspects of medicine, 24(6), 345-351. https://doi.org/10.1016/S0098-2997(03)00030-X.

Trouwborst, G., Oosterkamp, J., Hogewoning, S. W., Harbinson, J., & Van Ieperen, W. (2010). The responses of light interception, photosynthesis and fruit yield of cucumber to LEDâ€lighting within the canopy. Physiologia Plantarum, 138(3), 289-300.

Wang, J., Lu, W., Tong, Y., & Yang, Q. (2016). Leaf morphology, photosynthetic performance, chlorophyll fluorescence, stomatal development of lettuce (Lactuca sativa L.) exposed to different ratios of red light to blue light. Frontiers in plant science, 7, 250. https://doi.org/10.3389/fpls.2016.00250

Wellburn, A. R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of plant physiology, 144(3), 307-313.

Yeh, N., Ding, T. J., & Yeh, P. (2015). Light-emitting diodes׳ light qualities and their corresponding scientific applications. Renewable and Sustainable Energy Reviews, 51, 55-61. https://doi.org/10.1016/j.rser.2015.04.177.

Zheng, Y. J., Zhang, Y. T., Liu, H. C., Li, Y. M., Liu, Y. L., Hao, Y. W., & Lei, B. F. (2018). Supplemental blue light increases growth and quality of greenhouse pak choi depending on cultivar and supplemental light intensity. Journal of Integrative Agriculture, 17(10), 2245-2256.

Zhao, X., Qiao, X. R., Yuan, J., Ma, X. F., & Zhang, X. (2012). Nitric oxide inhibits blue light-induced stomatal opening by regulating the K+ influx in guard cells. Plant science, 184, 29-35.

Zhao, X., Zhao, Q., Xu, C., Wang, J., Zhu, J., Shang, B., & Zhang, X. (2018). Phot2â€regulated relocation of NPH3 mediates phototropic response to highâ€intensity blue light in Arabidopsis thaliana. Journal of integrative plant biology, 60(7), 562-577.

Zheng, L., He, H., & Song, W. (2019). Application of light-emitting diodes and the effect of light quality on horticultural crops: A review. HortScience, 54(10), 1656-1661.

Cover Vol 13 No 2 (2022)

Downloads

Published

01-10-2022

Issue

Vol. 13 No. 2 (2022)

Section

Articles

License

Copyright Notice

The author sends the manuscript with the understanding that if accepted for publication, the copyright of the article belongs to the author and retains publishing rights without restriction.

Copyright includes the non-exclusive right to reproduce and submit articles in all forms and media, including reprints, photographs, microfilm, and other similar reproductions, as well as translations. Reproduction of any part of this journal, its storage in the database and its transmission by all forms of media, such as electronic, electrostatic and mechanical copies, photocopying, recording, magnetic media, etc.

The journal editorial board makes every effort to ensure that no false or misleading data, opinions, or statements are published in the journal. Either way, the contents of articles and advertisements published in journals are the sole and exclusive responsibility of each writer and advertiser.

All articles published Open Access will be immediately and permanently free for everyone to read and download. We are continuously working with our author communities to select the best choice of license options, currently being defined for this journal as follows: Attribution 4.0 International (CC BY 4.0)