Pengaruh nisbah BAP dan IBA terhadap pembentukan embrio somatik pada tanaman Hoya carnosa (L.) R. Br.
DOI:
https://doi.org/10.35891/agx.v14i1.3262Keywords:
Regeneration, Somatic embryo, tendrils, HoyaAbstract
ð˜ð˜¯ð˜µð˜³ð˜°ð˜¥ð˜¶ð˜¤ð˜µð˜ªð˜°ð˜¯: ð˜ð˜°ð˜ºð˜¢ ð˜±ð˜ð˜¢ð˜¯ð˜µð˜´ ð˜¢ð˜³ð˜¦ 𘢠ð˜¸ð˜¦ð˜¢ð˜ð˜µð˜© ð˜°ð˜§ ð˜§ð˜ð˜°ð˜³ð˜¢ ð˜ªð˜¯ ð˜ð˜¯ð˜¥ð˜°ð˜¯ð˜¦ð˜´ð˜ªð˜¢. ð˜ð˜°ð˜ºð˜¢ ð˜ªð˜´ ð˜¶ð˜´ð˜¦ð˜¥ ð˜¢ð˜´ ð˜¢ð˜¯ ð˜°ð˜³ð˜¯ð˜¢ð˜®ð˜¦ð˜¯ð˜µð˜¢ð˜ ð˜±ð˜ð˜¢ð˜¯ð˜µ ð˜µð˜©ð˜¢ð˜µ ð˜©ð˜¢ð˜´ ð˜£ð˜¦ð˜¢ð˜¶ð˜µð˜ªð˜§ð˜¶ð˜ ð˜§ð˜ð˜°ð˜¸ð˜¦ð˜³ð˜´ ð˜¢ð˜¯ð˜¥ ð˜©ð˜ªð˜¨ð˜© ð˜¦ð˜¤ð˜°ð˜¯ð˜°ð˜®ð˜ªð˜¤ ð˜·ð˜¢ð˜ð˜¶ð˜¦. ð˜—ð˜³ð˜°ð˜±ð˜¢ð˜¨ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜µð˜©ð˜³ð˜°ð˜¶ð˜¨ð˜© ð˜ð˜¦ð˜¢ð˜§ ð˜¤ð˜¶ð˜µð˜µð˜ªð˜¯ð˜¨ð˜´ ð˜©ð˜¢ð˜´ ð˜¯ð˜°ð˜µ ð˜£ð˜¦ð˜¦ð˜¯ ð˜¢ð˜£ð˜ð˜¦ ð˜µð˜° ð˜±ð˜³ð˜°ð˜¥ð˜¶ð˜¤ð˜¦ ð˜´ð˜©ð˜°ð˜°ð˜µð˜´. ð˜›ð˜©ð˜¦ ð˜µð˜ªð˜´ð˜´ð˜¶ð˜¦ ð˜¤ð˜¶ð˜ð˜µð˜¶ð˜³ð˜¦ ð˜µð˜¦ð˜¤ð˜©ð˜¯ð˜°ð˜ð˜°ð˜¨ð˜º ð˜¤ð˜¢ð˜¯ ð˜±ð˜ð˜¢ð˜º 𘢠ð˜³ð˜°ð˜ð˜¦ ð˜¢ð˜´ ð˜ð˜°ð˜ºð˜¢ ð˜±ð˜³ð˜°ð˜±ð˜¢ð˜¨ð˜¢ð˜µð˜ªð˜°ð˜¯. ð˜—ð˜³ð˜°ð˜±ð˜¢ð˜¨ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜°ð˜§ ð˜µð˜©ð˜¦ ð˜¶ð˜´ð˜¦ ð˜°ð˜§ ð˜µð˜ªð˜´ð˜´ð˜¶ð˜¦ ð˜¤ð˜¶ð˜ð˜µð˜¶ð˜³ð˜¦ ð˜¤ð˜¢ð˜¯ ð˜£ð˜¦ ð˜µð˜©ð˜³ð˜°ð˜¶ð˜¨ð˜© ð˜ªð˜¯ð˜¥ð˜ªð˜³ð˜¦ð˜¤ð˜µ ð˜´ð˜°ð˜®ð˜¢ð˜µð˜ªð˜¤ ð˜¦ð˜®ð˜£ð˜³ð˜ºð˜°ð˜¨ð˜¦ð˜¯ð˜¦ð˜´ð˜ªð˜´ ð˜°ð˜³ ð˜µð˜©ð˜³ð˜°ð˜¶ð˜¨ð˜© ð˜¤ð˜¢ð˜ð˜ð˜¶ð˜´. ð˜šð˜°ð˜®ð˜¢ð˜µð˜ªð˜¤ ð˜¦ð˜®ð˜£ð˜³ð˜ºð˜° ð˜³ð˜¦ð˜¨ð˜¦ð˜¯ð˜¦ð˜³ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜£ð˜º ð˜¢ð˜¥ð˜¥ð˜ªð˜¯ð˜¨ ð˜µð˜©ð˜¦ ð˜³ð˜ªð˜¨ð˜©ð˜µ ð˜©ð˜°ð˜³ð˜®ð˜°ð˜¯ð˜¦ð˜´ ð˜¤ð˜¢ð˜¯ ð˜§ð˜°ð˜³ð˜® 𘢠ð˜¸ð˜©ð˜°ð˜ð˜¦ ð˜±ð˜ð˜¢ð˜¯ð˜µ. ð˜—ð˜¶ð˜³ð˜±ð˜°ð˜´ð˜¦: ð˜›ð˜©ð˜¦ ð˜¢ð˜ªð˜® ð˜°ð˜§ ð˜µð˜©ð˜¦ ð˜´ð˜µð˜¶ð˜¥ð˜º ð˜¸ð˜¢ð˜´ ð˜µð˜° ð˜¥ð˜¦ð˜µð˜¦ð˜³ð˜®ð˜ªð˜¯ð˜¦ ð˜µð˜©ð˜¦ ð˜¦ð˜§ð˜§ð˜¦ð˜¤ð˜µ ð˜°ð˜§ ð˜µð˜©ð˜¦ ð˜¤ð˜°ð˜®ð˜£ð˜ªð˜¯ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜°ð˜§ ð˜‰ð˜ˆð˜— ð˜¢ð˜¯ð˜¥ ð˜ð˜‰ð˜ˆ ð˜©ð˜°ð˜³ð˜®ð˜°ð˜¯ð˜¦ð˜´ ð˜°ð˜¯ ð˜µð˜©ð˜¦ ð˜§ð˜°ð˜³ð˜®ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜°ð˜§ ð˜´ð˜°ð˜®ð˜¢ð˜µð˜ªð˜¤ ð˜¦ð˜®ð˜£ð˜³ð˜ºð˜°ð˜´ ð˜ªð˜¯ ð˜©ð˜°ð˜ºð˜¢ ð˜¢ð˜´ 𘢠ð˜®ð˜¦ð˜µð˜©ð˜°ð˜¥ ð˜°ð˜§ ð˜©ð˜°ð˜ºð˜¢ ð˜±ð˜³ð˜°ð˜±ð˜¢ð˜¨ð˜¢ð˜µð˜ªð˜°ð˜¯. ð˜”ð˜¦ð˜µð˜©ð˜°ð˜¥ð˜´: ð˜›ð˜©ð˜ªð˜´ ð˜´ð˜µð˜¶ð˜¥ð˜º ð˜¶ð˜´ð˜¦ð˜¥ 𘢠ð˜´ð˜ªð˜¯ð˜¨ð˜ð˜¦ ð˜¤ð˜°ð˜®ð˜±ð˜ð˜¦ð˜µð˜¦ð˜ð˜º ð˜³ð˜¢ð˜¯ð˜¥ð˜°ð˜®ð˜ªð˜»ð˜¦ð˜¥ ð˜¥ð˜¦ð˜´ð˜ªð˜¨ð˜¯ ð˜¸ð˜ªð˜µð˜© 𘢠ð˜¤ð˜°ð˜®ð˜±ð˜¢ð˜³ð˜ªð˜´ð˜°ð˜¯ ð˜°ð˜§ ð˜‰ð˜ˆð˜— ð˜¢ð˜¯ð˜¥ ð˜ð˜‰ð˜ˆ ð˜©ð˜°ð˜³ð˜®ð˜°ð˜¯ð˜¦ð˜´ ð˜°ð˜¯ ð˜”𘚠ð˜®ð˜¦ð˜¥ð˜ªð˜¢. ð˜Œð˜¢ð˜¤ð˜© ð˜µð˜³ð˜¦ð˜¢ð˜µð˜®ð˜¦ð˜¯ð˜µ ð˜¸ð˜¢ð˜´ ð˜³ð˜¦ð˜±ð˜¦ð˜¢ð˜µð˜¦ð˜¥ 3 ð˜³ð˜¦ð˜±ð˜ð˜ªð˜¤ð˜¢ð˜µð˜ªð˜°ð˜¯ð˜´. ð˜™ð˜¦ð˜´ð˜¶ð˜ð˜µð˜´: ð˜‰ð˜¢ð˜´ð˜¦ð˜¥ ð˜°ð˜¯ ð˜µð˜©ð˜¦ ð˜³ð˜¦ð˜´ð˜¶ð˜ð˜µð˜´ ð˜°ð˜§ ð˜µð˜©ð˜¦ ð˜´ð˜µð˜¶ð˜¥ð˜º, ð˜µð˜©ð˜¦ ð˜¤ð˜°ð˜®ð˜£ð˜ªð˜¯ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜µð˜³ð˜¦ð˜¢ð˜µð˜®ð˜¦ð˜¯ð˜µ ð˜°ð˜§ 0.5 ð˜®ð˜¨/𘓠ð˜‰ð˜ˆð˜— ð˜¢ð˜¯ð˜¥ 1 ð˜®ð˜¨/𘓠ð˜ð˜‰ð˜ˆ ð˜¸ð˜¢ð˜´ ð˜µð˜©ð˜¦ ð˜£ð˜¦ð˜´ð˜µ ð˜¯ð˜¶ð˜®ð˜£ð˜¦ð˜³ ð˜°ð˜§ ð˜µð˜¦ð˜¯ð˜¥ð˜³ð˜ªð˜ð˜´ ð˜ªð˜¯ ð˜´ð˜°ð˜®ð˜¢ð˜µð˜ªð˜¤ ð˜¦ð˜®ð˜£ð˜³ð˜ºð˜° ð˜³ð˜¦ð˜¨ð˜¦ð˜¯ð˜¦ð˜³ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜¸ð˜ªð˜µð˜© ð˜¢ð˜¯ ð˜¢ð˜·ð˜¦ð˜³ð˜¢ð˜¨ð˜¦ ð˜°ð˜§ 40 ð˜µð˜¦ð˜¯ð˜¥ð˜³ð˜ªð˜ð˜´. ð˜›ð˜©ð˜¦ ð˜´ð˜ªð˜¯ð˜¨ð˜ð˜¦ ð˜‰ð˜ˆð˜— ð˜µð˜³ð˜¦ð˜¢ð˜µð˜®ð˜¦ð˜¯ð˜µ ð˜°ð˜§ 1 ð˜®ð˜¨/𘓠ð˜´ð˜©ð˜°ð˜¸ð˜¦ð˜¥ ð˜µð˜©ð˜¦ ð˜ð˜°ð˜¸ð˜¦ð˜´ð˜µ ð˜¢ð˜·ð˜¦ð˜³ð˜¢ð˜¨ð˜¦ ð˜¯ð˜¶ð˜®ð˜£ð˜¦ð˜³ ð˜°ð˜§ ð˜µð˜¦ð˜¯ð˜¥ð˜³ð˜ªð˜ð˜´ ð˜°ð˜§ 8.7 ð˜µð˜¦ð˜¯ð˜¥ð˜³ð˜ªð˜ð˜´. ð˜Šð˜°ð˜¯ð˜¤ð˜ð˜¶ð˜´ð˜ªð˜°ð˜¯: ð˜™ð˜¦ð˜¨ð˜¦ð˜¯ð˜¦ð˜³ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜°ð˜§ ð˜´ð˜°ð˜®ð˜¢ð˜µð˜ªð˜¤ ð˜¦ð˜®ð˜£ð˜³ð˜ºð˜°ð˜´ ð˜¸ð˜ªð˜µð˜© ð˜µð˜©ð˜¦ ð˜¤ð˜°ð˜®ð˜£ð˜ªð˜¯ð˜¢ð˜µð˜ªð˜°ð˜¯ ð˜°ð˜§ ð˜‰ð˜ˆð˜— ð˜¢ð˜¯ð˜¥ ð˜ð˜‰ð˜ˆ ð˜¸ð˜¢ð˜´ ð˜¢ð˜£ð˜ð˜¦ ð˜µð˜° ð˜¨ð˜³ð˜°ð˜¸ ð˜µð˜¦ð˜¯ð˜¥ð˜³ð˜ªð˜ð˜´, ð˜£ð˜¶ð˜µ ð˜´ð˜°ð˜®ð˜¢ð˜µð˜ªð˜¤ ð˜¦ð˜®ð˜£ð˜³ð˜ºð˜°ð˜´ ð˜¸ð˜¦ð˜³ð˜¦ ð˜¯ð˜°ð˜µ ð˜¢ð˜£ð˜ð˜¦ ð˜µð˜° ð˜ªð˜¯ð˜¥ð˜¶ð˜¤ð˜¦ ð˜´ð˜©ð˜°ð˜°ð˜µ ð˜¢ð˜¯ð˜¥ ð˜³ð˜°ð˜°ð˜µð˜´.
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