Pengaruh nisbah BAP dan IBA terhadap pembentukan embrio somatik pada tanaman Hoya carnosa (L.) R. Br.

Didik Pudji Restanto; Mohammad Candra Prayoga; Sigit Soeparjono; Riza Yuli  Rusdiana; Fathurrahman

doi:10.35891/agx.v14i1.3262

Didik Pudji Restanto Universitas Jember, Jl. Kalimantan no 37, Sumbersari, Jember 68121
Mohammad Candra Prayoga Universitas Jember, Jl. Kalimantan no 37, Sumbersari, Jember 68121
Sigit Soeparjono Universitas Jember, Jl. Kalimantan no 37, Sumbersari, Jember 68121
Riza Yuli Rusdiana Universitas Jember, Jl. Kalimantan no 37, Sumbersari, Jember 68121
Fathurrahman Universitas 17 Agustus 1945 Banyuwangi, Jl. Adi Sucipto 26 Banyuwangi

DOI: https://doi.org/10.35891/agx.v14i1.3262

Keywords: Regeneration, Somatic embryo, tendrils, Hoya

Abstract

𝘐𝘯𝘵𝘳𝘰𝘥𝘶𝘤𝘵𝘪𝘰𝘯: 𝘏𝘰𝘺𝘢 𝘱𝘭𝘢𝘯𝘵𝘴 𝘢𝘳𝘦 𝘢 𝘸𝘦𝘢𝘭𝘵𝘩 𝘰𝘧 𝘧𝘭𝘰𝘳𝘢 𝘪𝘯 𝘐𝘯𝘥𝘰𝘯𝘦𝘴𝘪𝘢. 𝘏𝘰𝘺𝘢 𝘪𝘴 𝘶𝘴𝘦𝘥 𝘢𝘴 𝘢𝘯 𝘰𝘳𝘯𝘢𝘮𝘦𝘯𝘵𝘢𝘭 𝘱𝘭𝘢𝘯𝘵 𝘵𝘩𝘢𝘵 𝘩𝘢𝘴 𝘣𝘦𝘢𝘶𝘵𝘪𝘧𝘶𝘭 𝘧𝘭𝘰𝘸𝘦𝘳𝘴 𝘢𝘯𝘥 𝘩𝘪𝘨𝘩 𝘦𝘤𝘰𝘯𝘰𝘮𝘪𝘤 𝘷𝘢𝘭𝘶𝘦. 𝘗𝘳𝘰𝘱𝘢𝘨𝘢𝘵𝘪𝘰𝘯 𝘵𝘩𝘳𝘰𝘶𝘨𝘩 𝘭𝘦𝘢𝘧 𝘤𝘶𝘵𝘵𝘪𝘯𝘨𝘴 𝘩𝘢𝘴 𝘯𝘰𝘵 𝘣𝘦𝘦𝘯 𝘢𝘣𝘭𝘦 𝘵𝘰 𝘱𝘳𝘰𝘥𝘶𝘤𝘦 𝘴𝘩𝘰𝘰𝘵𝘴. 𝘛𝘩𝘦 𝘵𝘪𝘴𝘴𝘶𝘦 𝘤𝘶𝘭𝘵𝘶𝘳𝘦 𝘵𝘦𝘤𝘩𝘯𝘰𝘭𝘰𝘨𝘺 𝘤𝘢𝘯 𝘱𝘭𝘢𝘺 𝘢 𝘳𝘰𝘭𝘦 𝘢𝘴 𝘏𝘰𝘺𝘢 𝘱𝘳𝘰𝘱𝘢𝘨𝘢𝘵𝘪𝘰𝘯. 𝘗𝘳𝘰𝘱𝘢𝘨𝘢𝘵𝘪𝘰𝘯 𝘰𝘧 𝘵𝘩𝘦 𝘶𝘴𝘦 𝘰𝘧 𝘵𝘪𝘴𝘴𝘶𝘦 𝘤𝘶𝘭𝘵𝘶𝘳𝘦 𝘤𝘢𝘯 𝘣𝘦 𝘵𝘩𝘳𝘰𝘶𝘨𝘩 𝘪𝘯𝘥𝘪𝘳𝘦𝘤𝘵 𝘴𝘰𝘮𝘢𝘵𝘪𝘤 𝘦𝘮𝘣𝘳𝘺𝘰𝘨𝘦𝘯𝘦𝘴𝘪𝘴 𝘰𝘳 𝘵𝘩𝘳𝘰𝘶𝘨𝘩 𝘤𝘢𝘭𝘭𝘶𝘴. 𝘚𝘰𝘮𝘢𝘵𝘪𝘤 𝘦𝘮𝘣𝘳𝘺𝘰 𝘳𝘦𝘨𝘦𝘯𝘦𝘳𝘢𝘵𝘪𝘰𝘯 𝘣𝘺 𝘢𝘥𝘥𝘪𝘯𝘨 𝘵𝘩𝘦 𝘳𝘪𝘨𝘩𝘵 𝘩𝘰𝘳𝘮𝘰𝘯𝘦𝘴 𝘤𝘢𝘯 𝘧𝘰𝘳𝘮 𝘢 𝘸𝘩𝘰𝘭𝘦 𝘱𝘭𝘢𝘯𝘵. 𝘗𝘶𝘳𝘱𝘰𝘴𝘦: 𝘛𝘩𝘦 𝘢𝘪𝘮 𝘰𝘧 𝘵𝘩𝘦 𝘴𝘵𝘶𝘥𝘺 𝘸𝘢𝘴 𝘵𝘰 𝘥𝘦𝘵𝘦𝘳𝘮𝘪𝘯𝘦 𝘵𝘩𝘦 𝘦𝘧𝘧𝘦𝘤𝘵 𝘰𝘧 𝘵𝘩𝘦 𝘤𝘰𝘮𝘣𝘪𝘯𝘢𝘵𝘪𝘰𝘯 𝘰𝘧 𝘉𝘈𝘗 𝘢𝘯𝘥 𝘐𝘉𝘈 𝘩𝘰𝘳𝘮𝘰𝘯𝘦𝘴 𝘰𝘯 𝘵𝘩𝘦 𝘧𝘰𝘳𝘮𝘢𝘵𝘪𝘰𝘯 𝘰𝘧 𝘴𝘰𝘮𝘢𝘵𝘪𝘤 𝘦𝘮𝘣𝘳𝘺𝘰𝘴 𝘪𝘯 𝘩𝘰𝘺𝘢 𝘢𝘴 𝘢 𝘮𝘦𝘵𝘩𝘰𝘥 𝘰𝘧 𝘩𝘰𝘺𝘢 𝘱𝘳𝘰𝘱𝘢𝘨𝘢𝘵𝘪𝘰𝘯. 𝘔𝘦𝘵𝘩𝘰𝘥𝘴: 𝘛𝘩𝘪𝘴 𝘴𝘵𝘶𝘥𝘺 𝘶𝘴𝘦𝘥 𝘢 𝘴𝘪𝘯𝘨𝘭𝘦 𝘤𝘰𝘮𝘱𝘭𝘦𝘵𝘦𝘭𝘺 𝘳𝘢𝘯𝘥𝘰𝘮𝘪𝘻𝘦𝘥 𝘥𝘦𝘴𝘪𝘨𝘯 𝘸𝘪𝘵𝘩 𝘢 𝘤𝘰𝘮𝘱𝘢𝘳𝘪𝘴𝘰𝘯 𝘰𝘧 𝘉𝘈𝘗 𝘢𝘯𝘥 𝘐𝘉𝘈 𝘩𝘰𝘳𝘮𝘰𝘯𝘦𝘴 𝘰𝘯 𝘔𝘚 𝘮𝘦𝘥𝘪𝘢. 𝘌𝘢𝘤𝘩 𝘵𝘳𝘦𝘢𝘵𝘮𝘦𝘯𝘵 𝘸𝘢𝘴 𝘳𝘦𝘱𝘦𝘢𝘵𝘦𝘥 3 𝘳𝘦𝘱𝘭𝘪𝘤𝘢𝘵𝘪𝘰𝘯𝘴. 𝘙𝘦𝘴𝘶𝘭𝘵𝘴: 𝘉𝘢𝘴𝘦𝘥 𝘰𝘯 𝘵𝘩𝘦 𝘳𝘦𝘴𝘶𝘭𝘵𝘴 𝘰𝘧 𝘵𝘩𝘦 𝘴𝘵𝘶𝘥𝘺, 𝘵𝘩𝘦 𝘤𝘰𝘮𝘣𝘪𝘯𝘢𝘵𝘪𝘰𝘯 𝘵𝘳𝘦𝘢𝘵𝘮𝘦𝘯𝘵 𝘰𝘧 0.5 𝘮𝘨/𝘓 𝘉𝘈𝘗 𝘢𝘯𝘥 1 𝘮𝘨/𝘓 𝘐𝘉𝘈 𝘸𝘢𝘴 𝘵𝘩𝘦 𝘣𝘦𝘴𝘵 𝘯𝘶𝘮𝘣𝘦𝘳 𝘰𝘧 𝘵𝘦𝘯𝘥𝘳𝘪𝘭𝘴 𝘪𝘯 𝘴𝘰𝘮𝘢𝘵𝘪𝘤 𝘦𝘮𝘣𝘳𝘺𝘰 𝘳𝘦𝘨𝘦𝘯𝘦𝘳𝘢𝘵𝘪𝘰𝘯 𝘸𝘪𝘵𝘩 𝘢𝘯 𝘢𝘷𝘦𝘳𝘢𝘨𝘦 𝘰𝘧 40 𝘵𝘦𝘯𝘥𝘳𝘪𝘭𝘴. 𝘛𝘩𝘦 𝘴𝘪𝘯𝘨𝘭𝘦 𝘉𝘈𝘗 𝘵𝘳𝘦𝘢𝘵𝘮𝘦𝘯𝘵 𝘰𝘧 1 𝘮𝘨/𝘓 𝘴𝘩𝘰𝘸𝘦𝘥 𝘵𝘩𝘦 𝘭𝘰𝘸𝘦𝘴𝘵 𝘢𝘷𝘦𝘳𝘢𝘨𝘦 𝘯𝘶𝘮𝘣𝘦𝘳 𝘰𝘧 𝘵𝘦𝘯𝘥𝘳𝘪𝘭𝘴 𝘰𝘧 8.7 𝘵𝘦𝘯𝘥𝘳𝘪𝘭𝘴. 𝘊𝘰𝘯𝘤𝘭𝘶𝘴𝘪𝘰𝘯: 𝘙𝘦𝘨𝘦𝘯𝘦𝘳𝘢𝘵𝘪𝘰𝘯 𝘰𝘧 𝘴𝘰𝘮𝘢𝘵𝘪𝘤 𝘦𝘮𝘣𝘳𝘺𝘰𝘴 𝘸𝘪𝘵𝘩 𝘵𝘩𝘦 𝘤𝘰𝘮𝘣𝘪𝘯𝘢𝘵𝘪𝘰𝘯 𝘰𝘧 𝘉𝘈𝘗 𝘢𝘯𝘥 𝘐𝘉𝘈 𝘸𝘢𝘴 𝘢𝘣𝘭𝘦 𝘵𝘰 𝘨𝘳𝘰𝘸 𝘵𝘦𝘯𝘥𝘳𝘪𝘭𝘴, 𝘣𝘶𝘵 𝘴𝘰𝘮𝘢𝘵𝘪𝘤 𝘦𝘮𝘣𝘳𝘺𝘰𝘴 𝘸𝘦𝘳𝘦 𝘯𝘰𝘵 𝘢𝘣𝘭𝘦 𝘵𝘰 𝘪𝘯𝘥𝘶𝘤𝘦 𝘴𝘩𝘰𝘰𝘵 𝘢𝘯𝘥 𝘳𝘰𝘰𝘵𝘴.

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