Pengaruh varietas dan konsentrasi broth kulit pisang sebagai media alternatif pertumbuhan Aspergillus niger

  • Nucky Istiqomah Mulyawati Universitas Yudharta Pasuruan, Indonesia
  • Muh. Aniar Hari Swasono Universitas Yudharta Pasuruan
  • Deny Utomo Universitas Yudharta Pasuruan, Indonesia
Keywords: Banana peel, Aspergillus niger, Alternative media growth

Abstract

Banana is an influential agriculture commodity in Indonesia, for consuming the fruit or making use of the leafs and steam, with high demand of banana fruit, there’s also left with tons of peel waste. Banana peel waste which usually contain cellulose (35 %–50 %), hemicellulose (25 %–30 %), and lignin (25 %–30 %). The fruit peel wastes containing sugars that are metabolized by microorganisms which make the potential of microbiology growth. Aspergillus niger as one of influental fungi for industrial can be used on production of α-Amylase. The experimental design used was Completely Randomized Design factorial with two factors. Factor I is Banana peel variety, with 4 level which contain ( Ambon Banana Peel, Kepok Banana Peel, Raja Banana Peel and Candi Banana Peel. Factor II is broth media concentration with 3 level ( 2gr banana peel : 100ml Aquadest ; 4gr banana peel : 100ml Aquadest; 6gr banana peel : 100ml Aquadest) with the result 12 combinations with 3 repeat. The result of the streak will be observed for seven days. The result of analysis will be analyzed with Analysis of Variance (ANOVA), if there’s a significant result, the analysis will be continued to LSD test 5%. The result of research pointed that variance of banana peel and concentration of broth media did show significant difference according to ANOVA result on day one until seven. Best variance of treatment found in V2K3 (Kepok Banana Peel with 6gr / 100ml concentration) resulted with average diameter reaching 55mm.

References

Ali, S. M., Pervaiz, A., Afzal, B., Hamid, N., & Yasmin, A. (2014). Open dumping of municipal solid waste and its hazardous impacts on soil and vegetation diversity at waste dumping sites of Islamabad city. Journal of King Saud University-Science, 26(1), 59-65.

Ali, S., Haq, I. U., Qadeer, M. A., & Iqbal, J. (2002). Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor. Electronic Journal of Biotechnology, 5(3), 19-20.

Anbu, S., Padma, J., Punithavalli, K., & Saranraj, P. (2017). Fruits peel waste as a novel media for the growth of economically important Fungi. Journal of Pharmacognosy and Phytochemistry, 6(6), 426-428.

Barnett, H.L., and B.B. Hunter. (1998). Illustrated Genera of Imperfect Fungi, 4th Edition. Macmillian Publishing Company, New York, NY.218.

Behera, S. S., & Ray, R. C. (2016). Solid state fermentation for production of microbial cellulases: recent advances and improvement strategies. International journal of biological macromolecules, 86, 656-669.

Deivanayaki, M., & Antony, I. P. (2012). Alternative vegetable nutrient source for microbial growth. International Journal of Biosciences (IJB), 2(5), 47-51.

Gandjar, Irawati., Sjamsuridzal Wellyzar., dan Oetari Ariyanti. (2006). Mikologi dasar dan terapan. Jakarta: Yayasan Obor Indonesia

Haq,I.,Ali,S.,Qadeer,M.A.and Iqbal,J. (2004). Citric acid production by mutants of Aspergillus niger from cane molasses. Bioresource Technology, 93(2), 125-130.

Jukofsky, & Dione. (2010). Encyclopedia of Rainforests, Banana (Musa acuminata). Connecticut: Oryx Press.

Kareem, S. O., Akpan, I., & Alebiowu, O. O. (2010). Production of citric acid by Aspergillus niger using pineapple waste. Malaysian Journal of Microbiology, 6(2), 161-165.

Kim, S. W., Jung, J. H., Lamsal, K., Kim, Y. S., Min, J. S., & Lee, Y. S. (2012). Antifungal effects of silver nanoparticles (AgNPs) against various plant pathogenic fungi. Mycobiology, 40(1), 53-58.

Kurniati, C. (2011). Pengaruh metodepengolahan kulit pisang batu (Musa brachyarpa) terhadap kandungan NDF, ADF, Selulosa, Hemiselulosa, Lignindan Silika. Skripsi. UniversitasAndalas. Padang.

Krishna, P. R., Srivastava, A. K., Ramaswamy, N. K., Suprasanna, P., & D’souza, S. F. (2012). Banana peel as substrate for α-amylase production using Aspergillus niger NCIM 616 and process optimization. IJBT, 11(3), 314-319.

Koni, TNI, Paga A, Foenay TA. (2006). Substitusi jagung dengan campuran kulit pisang dan ampas kelapa dalam ransum ayam pedaging. Laporan Hasil Penelitian Politani. Kupang.

Kwoseh, C.K., Darko. M. A., & Adubofour, K. (2012). Cassava Starch-Agar Blend as Alternative Gelling Agent For Mycological Culture Media. Bots. J. AgricApplSci, 8(1), 8- 15.

Martyniuk, Stefan O., & Jadwiga. (2011). Use of potato extract broth for culturing root-nodule bacteria. Polish Journal of Microbiology, 60 (4), 323–327.

Meletiadis, J., Meis, J. F., Mouton, J. W., & Verweij, P. E. (2001). Analysis of growth characteristics of filamentous fungi in different nutrient media. Journal of Clinical Microbiology, 39(2), 478-484.

Oladiji, A. T., Yakubu, M. T., Idoko, A. S., Adeyemi, O., & Salawu, M. O. (2010). Studies on the physicochemical properties and fatty acid composition of the oil from ripe plantain peel (Musa paradisiaca). African Scientist, 11(1), 73-78.

Ravimannan, N., Arulanantham, R., Pathmanathan, S., & Kularajani, N. (2014). Alternative Culture Media For Fungal Growth Using Different Formulation Of Protein Sources. Annals of Biological Research, 5(1), 36-39.

Suganthi, R., Benazir, J. F., Santhi, R., Ramesh, K.V., Anjana, H., Nitya M., Nidhiya, K. A., Kavitha, G., Lakshmi., R. (2011). Amylase Production By Aspergillus niger Under Solid State Fermentation Using Agro industrial Wastes. International Journal of Engineering Science and Technology (IJEST), 3(2), 1756-1763.

Saheed, O. K., Jamal, P., Karim, M. I. A., Alam, Z., & Muyibi, S. A. (2013). Cellulolytic fruits wastes: a potential support for enzyme assisted protein production. Journal of Biological Sciences, 13(5), 379-385.

Saranraj, P., & Anbu, S. (2017). Utilization of agroindustrial wastes for the cultivation of industrially important fungi – a review. International Journal of Innovations in Agricultural Sciences (IJIAS), 1(2), 59 – 71.

Schuster, E., Dunn-Coleman, N., Frisvad, J. C., & Van Dijck, P. W. (2002). On the safety of Aspergillus niger–a review. Applied microbiology and biotechnology, 59(4-5), 426-435.

Tharmila, S., Jeyaseelan, E. C., & Thavaranjit, A. C. (2011). Preliminary Screening Of Alternative Culture Media For The Growth Of Some Selected Fungi. Archives of Applied Science Research, 3(3), 389-393.

Published
2019-09-17
How to Cite
Mulyawati, N. I., Swasono, M. A. H., & Utomo, D. (2019). Pengaruh varietas dan konsentrasi broth kulit pisang sebagai media alternatif pertumbuhan Aspergillus niger. AGROMIX, 10(2), 114-129. https://doi.org/10.35891/agx.v10i2.1578
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Articles