Kadar klorofil ekstrak daun pepaya pada jenis pelarut dan kadar zat penstabil yang berbeda

Authors

  • Poppy Diana Sari Program Studi Teknologi Pangan, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur
  • Lukman Hudi Program Studi Teknologi Pangan, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur
  • Al - Machfud Program Studi Teknologi Pangan, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur
  • Aisyah Nurkhaliza Program Studi Teknologi Pangan, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur
  • Erika Puspita Sari Program Studi Teknologi Pangan, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur

DOI:

https://doi.org/10.35891/rdraga58

Keywords:

Chlorophyll, papaya leaves, extraction, solvent, NaHCO3

Abstract

Chlorophyll is a green coloring agent and antioxidant from plants that is needed in everyday life, both in the food industry, cosmetics and renewable energy. Its abundant presence in nature makes it interesting to research and develop its use. Various methods are used to extract chlorophyll from existing natural materials. One type of plant with the highest chlorophyll content is papaya leaves, but the highest chlorophyll content has not been found from the extraction process used so far. The use of papaya leaves has only been used for animal feed so it is very interesting to develop in order to be used further in other things. This study aims to obtain the highest chlorophyll content with the treatment of solvent types and levels of stabilizers used in the papaya leaf chlorophyll extraction process. The study was conducted with 2 treatment variables, including the type of solvent, namely ethanol and seton and the treatment of the levels of NaHCO3 stabilizers used, namely 1%, 3% and 5%. From the research that has been done, the highest results were obtained in the treatment using acetone as a solvent and the addition of NaHCO3 stabilizer as much as 3%. This treatment resulted in an extract yield of 7.56%, an average total chlorophyll content of 10.597%, an average chlorophyll a content of 5.916% and an average chlorophyll b content of 4.684%.

References

Abdilah, F., Indah, R., & Ahyar, A. (2014). Pengujian daya antioksidan dan sifat toksisitas ekstrak Co(II) turunan klorofil. Jurnal MIPA.

Aryanti, N., Nafiunisa, A., & Willis, F. M. (2016). Ekstraksi dan karakterisasi klorofil dari daun suji (Pleomele angustifolia) sebagai pewarna pangan alami. Jurnal Aplikasi Teknologi Pangan, 5(4). https://doi.org/10.17728/jatp.183

El-Mouhty, R. A. N., & El-Naggar, A. Y. (2014). Extraction of chlorophyll and carotene from irradiated parsley. International Journal of Innovative Research in Science, Engineering and Technology, 3(1), 8522–8527.

Hörtensteiner, S., & Kräutler, B. (2011). Chlorophyll breakdown in higher plants. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1807(8), 977–988. https://doi.org/10.1016/j.bbabio.2010.12.007

Hosikian, A., Lim, S., Halim, R., & Danquah, M. K. (2010). Chlorophyll extraction from microalgae: A review on the process engineering aspects. International Journal of Chemical Engineering, 1–11.

Hu, X., Tanaka, A., & Tanaka, R. (2013). Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples. Plant Methods, 9, 19–31.

Kong, W., Liu, N., Zhang, J., Yang, Q., Hua, S., Song, H., & Xia, C. (2014). Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology. Journal of Food Science and Technology, 51(9), 2006–2013.

Kurniawan, M. P., Widodo, F. M., & Agustini, T. W. (2013). Pengaruh penambahan MgCO₃ dan NaHCO₃ dengan perbedaan pencahayaan terhadap stabilitas pigmen klorofil-a mikroalga Chlorella vulgaris. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 2(3), 25–33.

Mukhriani. (2014). Ekstraksi, pemisahan senyawa, dan identifikasi senyawa aktif. Jurnal Kesehatan, 7(2).

Pumilia, G., Cichon, M. J., Cooperstone, J. L., Giuffrida, D., Dugo, G., & Schwartz, S. J. (2014). Changes in chlorophylls, chlorophyll degradation products, and lutein in pistachio kernels (Pistacia vera L.) during roasting. Food Research International, 65, 193–198. https://doi.org/10.1016/j.foodres.2014.05.047

Putri, W. D. R., Zubaidah, E., & Sholahudin, N. (2012). Ekstraksi pewarna alami daun suji: Kajian pengaruh blanching dan jenis bahan pengekstrak. Jurnal Teknologi Pertanian, 4(1), 13–24.

Sari, P. D., Wijaya, I. M. A. S., Giriantari, I. A. D., Wartini, N. M., & Hartati, R. S. (2025). Chlorophyll and magnesium content of pepaya leaves extract (Carica papaya L.) at various extraction times. Journal of Agricultural Sciences – Sri Lanka, 20(1). https://doi.org/10.4038/jas.v20i1.10110

Setiari, N., & Nurchayati, Y. (2009). Eksplorasi kandungan klorofil pada beberapa sayuran hijau sebagai alternatif bahan dasar food supplement. Bioma, 11(1), 6–10. https://doi.org/10.14710/bioma.11.1.6-10

Singh, A., Singh, A. P., & Ramaswamy, H. S. (2015). Effect of processing conditions on quality of green beans subjected to reciprocating agitation thermal processing. Food Research International, 78, 424–432. https://doi.org/10.1016/j.foodres.2015.08.040

Troy, D. B. (2005). Remington: The science and practice of pharmacy. Lippincott Williams & Wilkins.

Wiyono, A. E., Amilia, W., Shasabilah, R. T., Mulyana, R. A., & Pramesti, V. O. (2023). Potensi daun singkong (Manihot esculenta Crantz) sebagai pewarna alami. Teknotan, 17(1). https://doi.org/10.24198/jt.vol17n1.4

Wu, J. H., Wang, S. Y., & Chang, S. T. (2002). Extraction and determination of chlorophylls from moso bamboo (Phyllostachys pubescens) culm. Journal of Bamboo and Rattan, 1(2), 171–180.

Yuniwati, M., Kusuma, A. W., & Yunanto, F. (2012). Optimasi kondisi proses ekstraksi zat pewarna dalam daun suji dengan pelarut etanol. Dalam Prosiding Seminar Nasional Aplikasi Sains dan Teknologi (SNAST) (pp. A257–A263).

Zhang, J., Han, C., & Liu, Z. (2009). Absorption spectrum estimating rice chlorophyll concentration: Preliminary investigations. Journal of Plant Breeding and Crop Science, 1(5), 223–229.

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Published

2026-03-31

Issue

Vol. 17 No. 1 (2026)

Section

Article

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Copyright (c) 2026 Poppy Diana Sari, Lukman Hudi, Al-Machfudz, Aisyah Nurkhaliza, Erika Puspita Sari

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