Penambahan pigmen alami yang disalut tepung agar untuk meningkatkan kekuatan antioksidan pada tempe

The addition of natural pigments coated with agar powder to increase the antioxidant power of tempeh

  • Aditya Surya Piansti Program Studi Biologi, Universitas Kristen Satya Wacana, Kota Salatiga, Jawa Tengah
  • Anggara Mahardika Program Studi Teknologi Pangan, Universitas Nasional Karangturi, Semarang, Jawa Tengah
  • Lusiawati Dewi Program Studi Biologi, Universitas Kristen Satya Wacana, Kota Salatiga, Jawa Tengah
Keywords: agar, antioksidan, fermentasi, pigmen


Background: Tempeh is a traditional Indonesian food that has a relatively low antioxidant activity. The addition of natural pigments is one solution to increase the antioxidant activity of tempeh. However, natural pigments are very susceptible to damage due to environmental factors, so it is necessary to coat with certain materials, such as agar powder to protect the pigments from damage.

Research purposes: The aim of the study to analyze the effect of adding natural pigments coated with agar powder on the antioxidant strength of tempeh.

Method: The sample used was tempeh with the addition of pigment powder uncoated and coated with 2%, 3%, 4% of agar, and tempeh without the addition of pigments as control. The antioxidant activity strength was analyzed using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) method at  fermentation time of 0 and 48 hours.

Results and Discussion: The results showed that the addition of pigments and fermentation time were able to significantly increase the antioxidant strength of tempeh compared to control where the IC50 value of the control at 0 hours of fermentation was 166,45±38,58 mg/ml which changed to 69,45±3,49 mg/ml after 48 hours of fermentation. Antioxidant activity were stronger in tempeh with addition of pigment where IC50 value of tempeh with uncoated pigments, coated pigments with 2%, 3%, and 4% of agar were 23,53±5,46 mg/ml, 21,91±6,02 mg/ml, 21,53±6,03 mg/ml, and 29,69±11,04 mg/ml, respectively. Nevertheless, the antioxidant activity strength from all treatments were considered as weak (IC50>200 µg /ml).

Conclusion: The addition of pigments uncoated and coated with agar powder and fermentation time were able to increase the antioxidant strength of tempeh. Coating treatment is also able to maintain the pigment color from degradation in the fermentation process.


Astuti, M., Eng, B., Meliala, A., & Med, B. (2000). Tempe, a nutritious and healthy food from Indonesia. Asia Pacific J Clin Nutr, 9(4), 322–325.

Barros, F. C.N., da Silvaa, D. C., Sombra, V. G., Maciel, J. S., Feitosa, J. P.A., Freitas, A. L.P., & de Paula, R. C. M. (2013). Structural characterization of polysaccharide obtained from red seaweed Gracilaria caudata (J Agardh). Carbohydrate Polymers, 92(1), 598–603.

Cempaka, L., Eliza, N., Ardiansyah, A., Handoko, D. D., & Astuti, R. M. (2018). Proximate Composition, Total Phenolic Content, and Sensory Analysis of Rice Bran Tempeh. Makara Journal of Science, 22(2).

Chang, C. T., Hsu, C. K., Chou, S. T., Chen, Y. C., Huang, F. S., & Chung, Y. C. (2009). Effect of fermentation time on the antioxidant activities of tempeh prepared from fermented soybean using Rhizopus oligosporus. International Journal of Food Science and Technology, 44(4), 799–806.

Egarani, G. R., Kasmiyati, S., & Kristiani, E. B. K. (2020). The Antioxidant Content and Activity of Various Plant Organs of Kitolod ( Isotoma longiflora ). Journal of Biology & Biology Education,12(3), 297–303.

Ervina, M., Nawu, Y. E., & Esar, S. Y. (2016). Comparison of in vitro antioxidant activity of infusion , extract and fractions of Indonesian Cinnamon (Cinnamomum burmannii) bark. International Food Research Journal, 23(3), 1346–1350.

Hashim, N., Tai, C. W., Wen, H. X., Ismail, A., & Kong, K. W. (2018). Current Research in Nutrition and Food Science Comparative Evaluation of Antioxidant Properties and Isoflavones of Tempeh Fermented in Two Different Wrapping Materials. Current Research in Nutrition and Food Science, 6(2).

Kalita, T., Sangma, S. D., Bez, G., & Ambasht, P. K. (2020). Immobilization of Acid Phosphatase in Agar-agar and Gelatin: Comparative Characterization. Journal of Scientific Research, 64(02), 192–200.

Nout, M. J. R., & Kiers, J. L. (2005). Tempe fermentation, innovation and functionality: Update into the third millenium. Journal of Applied Microbiology, 98(4), 789-805.

Paciulli, M., Medina-Meza, I. G., Chiavaro, E., & Barbosa-Cánovas, G. V. (2016). Impact of thermal and high pressure processing on quality parameters of beetroot (Beta vulgaris L.). LWT - Food Science and Technology, 68, 98–104.

Phaniendra, A., Jestadi, D. B., & Periyasamy, L. (2015). Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Ind J Clin Biochem, 30(1), 11–26.

Puspita, D., & Samalukang, Y. (2017). Termostabilitas Antosianin dari Buah Basella rubra yang Dimikroenkapsulasi. Jurnal Teknologi Pangan dan Hasil Pertanian, 12(2), 29–38.

Rehman, H. U., Aman, A., Zohra, R. R., & Qader, S. A. U. (2014). Immobilization of pectin degrading enzyme from Bacillus licheniformis KIBGE IB-21 using agar-agar as a support. Carbohydrate Polymers, 102(1), 622–626.

Rhein-knudsen, N., Ale, M. T., & Meyer, A. S. (2015). Seaweed Hydrocolloid Production: An Update on Enzyme. Marine Drugs, 13(6), 3340-3359.

Saefudin, Marusin, S., & Chairul. (2013). Aktivitas Antioksidan pada Enam Jenis Tumbuhan Sterculiaceae. Jurnal Penelitian Hasil Hutan, 31(2), 103–109.

Sailaja Rao, P., Kalva, S., Yerramilli, A., & Mamidi, S. (2011). Free Radicals and Tissue Damage: Role of Antioxidants. Free Radicals and Antioxidants, 1(4), 2–7.

Sharma, M., Sharma, V., & Majumdar, D. K. (2014). Entrapment of α -Amylase in Agar Beads for Biocatalysis of Macromolecular Substrate. International Scholarly Research Notices, 1–8.

Widoyo, S., Handajani, S., & Nandariyah. (2015). Pengaruh lama fermentasi terhadap kadar serat kasar dan aktivitas antioksidan tempe beberapa varietas kedelai. Biofarmasi, 13(2), 59–65.

How to Cite
Piansti, A. S., Mahardika, A., & Dewi, L. (2021). Penambahan pigmen alami yang disalut tepung agar untuk meningkatkan kekuatan antioksidan pada tempe. Teknologi Pangan : Media Informasi Dan Komunikasi Ilmiah Teknologi Pertanian, 12(2), 231-238.